CONSERVE ENERGY REDUCE FATIGUE Fatigue is any tiredness and decrease in athletic performance that occurs during both high intensity exercise for short periods of time and lower intensity exercise. Much of our knowledge of fatigue in animals comes from horses because they can be readily trained to exercise on high-speed treadmills; allowing for controlled investigation of respiratory, cardiovascular, and metabolic responses. When fatigue occurs, changes in the horse’s gait, joint movements, muscular support, and willingness to perform over prolonged periods of time. When a horse is fatigued, he typically has the inability to continue exercise at a given level of intensity. Generally, the more intense the exercise, the earlier the onset of fatigue. Generally, exercise at a horse's highest attainable speed can't be maintained for more than 30-40 seconds. After that, fatigue sets in and the horse slows down. However, the cause of fatigue during exercise isn't the same for all horses or all events. For example, endurance horses competing over many hours or days experience fatigue, but the underlying mechanisms for fatigue is different than horses racing at maximal speeds lasting 3 minutes or less. According to Dr. David Marlin, the use of the word “fatigue” in relation to exercise has very specific meanings and covers a wide range of manifestations including: The horse that will not move another step The endurance horse at the end of a 100 mile race that is reluctant to trot The racehorse that has slowed in the last eighth of a mile of a race, but when passing the post is still travelling in excess of 35 miles per hour The event horse on cross-country that slows down from a fast gallop pace, but when given a few strides at a slightly slower pace, is able to return to the original faster pace REDUCE FATIGUE IN EXERCISING HORSES Horses wearing FLAIR® Strips have been shown to use 5-6% less energy when bringing air in during intensive exercise. Early research on horses exercising on treadmills showed that horses using FLAIR Strips used 5-6% less oxygen and produced less carbon dioxide than when doing the same amount of work without a FLAIR Strip. Using less oxygen means the horse consumed less energy. It's believed that by reducing resistance to airflow (allowing horses to breathe easier), the work required of the respiratory muscles like the diaphragm during exercise is reduced, which reduces the energy required for breathing. It was theorized that by reducing energy consumption, fatigue would be delayed since this energy would now be available to the exercising muscles. This delays the onset of time to fatigue, helps horses fatigue less and conserve energy so there's more available for tomorrow. This conserved energy helps sustain performance longer and potentially reduce the chance of fatigue related injuries. When fatigue occurs, the horse’s gait, joint movements, muscular support, and willingness to perform can change. These changes can contribute to structural fatigue injuries of the musculoskeletal system, such as pulled suspensory and other ligaments, bowed tendons and fractured bones. The energy conserved by wearing a FLAIR Strip is available to sustain performance longer and potentially reduce the chance of fatigue related injuries. The Strips are also beneficial during shorter duration, sprint exercises (1/4 mile races or barrel runs), where 60% of energy is generated by anaerobic metabolism and 40% of the energy is generated via aerobic pathways that require oxygen. However, at races over 5 furlongs (1000 meters) over 70% of energy is generated via aerobic pathways and at a mile (1600 meters), 80% of energy is generated via aerobic pathways. During longer distances even more energy is generated using aerobic pathways, making efficient oxygen intake even more important. Oxygen is also very important in the recovery phase following intensive exercise to rebuild energy stores consumed during exercise. The benefits that FLAIR Strips provide for efficient oxygen intake during intensive exercise continues through the recovery phase to continue providing efficient oxygen intake to rebuild energy stores. This is one more reason why it's important to not remove the Strip until the horse is fully cooled out and breathing has returned to normal. One of the major causes of fatigue is the depletion of energy stores needed for the horse to perform. During less intense, longer duration exercise, FLAIR Strips help horses get needed oxygen more efficiently to sustain aerobic exercise. CAUSES OF FATIGUE Fatigue can result from the failure of one enzyme system, one cell, one organ or one body system, but is more often due to multiple factors contributing simultaneously. This can place an excessive burden on other body systems as they try to compensate. In many ways, fatigue is still poorly understood. However, it's generally accepted that no single mechanism explains all the different aspects of fatigue that are recognized. For example, a dramatic reduction of muscle glycogen can play a significant role in fatigue at the end of an endurance ride. But, fatigue also occurs when muscle glycogen concentrations are still high, for example in a show-jumper after completing its round. Factors unrelated to the intensity, duration or pattern of exercise influence the onset of fatigue, including: Metabolic myopathies (tying up and polysaccharide storage myopathy), over-training, fitness, age, body condition and environmental conditions (temperature, humidity, pollution or altitude). Numerous areas have been studied to try and understand the mechanisms of fatigue during exercise including: Depletion of the energy generating systems inside and outside muscle cells Accumulation of metabolic by-products (i.e. lactic acid, ammonia, H+ ions, and ATP metabolites) and failure of the muscular contractile mechanism Disturbances to acid-base, electrolyte, hydration, and thermoregulatory homeostasis Central and peripheral nervous system fatigue DEPLETION OF ENERGY STORES The depletion of energy is one of the major causes of fatigue. In the body, the fundamental source of energy at the cellular level is a molecule called ATP (adenosine triphosphate). ATP supports almost all cellular-based active processes, including muscle contraction, by breaking down ATP to ADP (adenosine diphosphate). However, the amount of ATP stored in the muscles is low and will only support exercise for several seconds of muscular activity. When ATP is broken down to ADP it must be regenerated back to ATP. There are four different ways to regenerate ATP from ADP: ATP Replenishment from Other High Energy Phosphate Molecules in the CellsThis process doesn't require oxygen and is often referred to as anaerobic energy production. ATP Replenishment from Glycogen Breakdown to Lactic AcidThis form of energy replenishment is also anaerobic (without using oxygen). It's the rapid breakdown of intramuscular energy stores of glycogen to lactic acid. Accumulation of lactic acid, hydrogen ions and ATP metabolites within muscles, and the resulting reduction is muscle pH and significantly increased potassium in the blood, which is believed to play a role in the development of fatigue. ATP Replenishment from Aerobic Metabolism of Muscle Glycogen or Blood GlucoseThe complete aerobic breakdown of glycogen or blood glucose to carbon dioxide and water requires oxygen and occurs mainly in the mitochondria of the cells. It's within the mitochondria that the oxygen is used and the majority of ATP is regenerated. This process is much more efficient at regenerating ATP from ADP, but is slower than the anaerobic pathways described above. Around 90% of the total body stores of glycogen are stored within the muscles, with most of the remainder being stored in the liver. ATP Replenishment from Aerobic Metabolism of FatThe aerobic metabolism of triglycerides (fat) to regenerate ATP requires oxygen and takes place primarily within mitochondria. It also generates carbon dioxide and water as the end products. In contrast to glycogen, triglycerides stored in the muscle account for only around 10% of the total body stores, with the remainder being found in adipose tissue and subcutaneous fat depots. Triglycerides are broken down using oxygen to liberate free fatty acids (FFAs), which are transported in the blood to be taken up by working muscles. While it's possible to deplete muscle glycogen stores, it's almost impossible to deplete stores of fat in a single bout of exercise. Repletion of muscle and liver glycogen in horses may take 24-72 hours. Depletion of brain glycogen has also been shown to occur in association with prolonged exercise, which has been suggested to be involved in central fatigue.